Part:BBa_K4574006
Anti-toxin A IGVL
Variable light chain locus of the AR-301 monoclonal IgG1 antibody, which specifically targets toxin A (hemolysin) of Staphylococcus aureus. This component is integral to tosatoxumab, a therapeutic antibody used against infections caused by MRSA.
Source
The following basic part was developed for the therapeutic monoclonal antibody against S. aureus toxin A by Rudolf et al.[1].
The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.
Usage and Biology
This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Rudolf, M., & Koch, H. (2011). Human monoclonal antibody against S. aureus derived alpha-toxin and its use in treating or preventing abscess formation (World Intellectual Property Organization Patent WO2011018208A1). https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2011018208
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